People

Sally Horne-Badovinac, PhD

My lab takes an integrative approach to studying organ morphogenesis. We are currently probing how cells migrate collectively & how basement membrane ECMs are assembled.



Collective cell migration - The collective migration of cells within an epithelial sheet underlies tissue remodeling events associated with morphogenesis, wound repair, and the spread of many cancers. Yet little is known about how each epithelial cell coordinates its individual migratory behaviors with those of its neighbors, or how epithelial motility shapes tissues during development. We recently discovered a novel planar signaling system that coordinates leading and trailing edge dynamics between neighboring epithelial cells, and are now working to elucidate the mechanistic basis of this signaling. We are also investigating how collective cell migration can be harnesses to create a stable, planar tissue pattern within an epithelium for subsequent morphogenesis.



Basement membrane dynamics – Basement membrane is a sheet-like extracellular matrix that lines the basal surface of all epithelial tissues. This complex protein network provides structural stability to the monolayer, promotes cell-cell and cell-matrix signaling, and acts as a physical barrier to metastasis. Despite their ubiquity and many essential functions, we know surprisingly little about how basement membranes are built or how they are remodeled during development. We are taking a comprehensive approach to identifying: the cellular mechanisms underlying basement membrane biosynthesis and secretion, how basement membrane biosynthetic programs are modulated to construct diverse matrix architectures, and how different basement membrane architectures ultimately influence organ morphogenesis.



Experimental system – We perform these studies in the Drosophila egg chamber, which is an organ-like structure in the ovary that will give rise to one egg. Each egg chamber is comprised of a germ cell cluster, surrounded by a somatic epithelium of follicle cells. The follicle cells produce their own basement membrane, which ensheaths the organ. Though initially spherical, egg chambers lengthen as they mature. This morphogenesis depends on a dramatic collective migration of the follicle cells across their basement membrane, which causes the entire egg chamber to rotate within its surrounding matrix. Importantly, the follicle cells also remodel the basement membrane as they crawl along its surface, creating a polarized array of fibrils in the direction of tissue movement. This fibrillar matrix is then thought to act as a “molecular corset” that resists the expansive growth of the germ cells to directionally bias egg chamber growth, and thus create the elongated shape of the egg. A major strength of this system is that the collective migration and basement membrane remodeling both occur on the egg chamber’s outer surface, which allows us to image dynamic cell behaviors - in a living organ - with exquisite clarity. Moreover, we can use the sophisticated genetic tools of Drosophila to identify the underlying molecular mechanisms. Please check out the papers below to see what we have already discovered!

UC Berkeley
Postdoc - Cell and Developmental Biology
2008

UC San Francisco
PhD - Biochemistry
2003

University of Oregon
BS - Chemistry
1996

ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.
Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz AP, Horne-Badovinac S, Green WN, Glick BS. ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms. Mol Biol Cell. 2020 12 15; 31(26):2892-2903.
PMID: 33112725

The Drosophila micropyle as a system to study how epithelia build complex extracellular structures.
Horne-Badovinac S. The Drosophila micropyle as a system to study how epithelia build complex extracellular structures. Philos Trans R Soc Lond B Biol Sci. 2020 10 12; 375(1809):20190561.
PMID: 32829690

Mobilizing the Matrix for Organ Morphogenesis.
Horne-Badovinac S. Mobilizing the Matrix for Organ Morphogenesis. Dev Cell. 2020 07 06; 54(1):1-2.
PMID: 32634396

Looking deeper into tissue elongation.
Williams AM, Horne-Badovinac S. Looking deeper into tissue elongation. Nat Rev Mol Cell Biol. 2020 06; 21(6):305.
PMID: 32071437

Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation.
Cerqueira Campos F, Dennis C, Alégot H, Fritsch C, Isabella A, Pouchin P, Bardot O, Horne-Badovinac S, Mirouse V. Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation. Development. 2020 04 08; 147(7).
PMID: 32156755

Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation.
Cerqueira Campos F, Dennis C, Alégot H, Fritsch C, Isabella A, Pouchin P, Bardot O, Horne-Badovinac S, Mirouse V. Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation. Development. 2020 Jan 01.
PMID: 34004855

In-silico definition of the Drosophila melanogaster matrisome.
Davis MN, Horne-Badovinac S, Naba A. In-silico definition of the Drosophila melanogaster matrisome. Matrix Biol Plus. 2019 Nov; 4:100015.
PMID: 33543012

The dPix-Git complex is essential to coordinate epithelial morphogenesis and regulate myosin during Drosophila egg chamber development.
Dent LG, Manning SA, Kroeger B, Williams AM, Saiful Hilmi AJ, Crea L, Kondo S, Horne-Badovinac S, Harvey KF. The dPix-Git complex is essential to coordinate epithelial morphogenesis and regulate myosin during Drosophila egg chamber development. PLoS Genet. 2019 05; 15(5):e1008083.
PMID: 31116733

Planar-Polarized Semaphorin-5c and Plexin A Promote the Collective Migration of Epithelial Cells in Drosophila.
Stedden CG, Menegas W, Zajac AL, Williams AM, Cheng S, Özkan E, Horne-Badovinac S. Planar-Polarized Semaphorin-5c and Plexin A Promote the Collective Migration of Epithelial Cells in Drosophila. Curr Biol. 2019 03 18; 29(6):908-920.e6.
PMID: 30827914

Tissue Structure: A CIVICs Lesson for Adipocytes.
Zajac AL, Horne-Badovinac S. Tissue Structure: A CIVICs Lesson for Adipocytes. Curr Biol. 2017 09 25; 27(18):R1013-R1015.
PMID: 28950083

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Quantrell Award for Excellence in Undergraduate Teaching
The University of Chicago
2021

Basil O'Connor Starter Scholar Research Award
The March of Dimes Foundation
2010 - 2012

Young Investigator Award
Edward Mallinckrodt, Jr. Foundation
2009 - 2012

Postdoctoral Fellowship
Jane Coffin Childs Foundation
2003 - 2006

Harold C. Weintraub Graduate Student Award
Fred Hutchinson Cancer Center
2003

Predoctoral Fellowship
American Heart Association
2001 - 2003

Predoctoral Fellowship
National Science Foundation
1998 - 2001

American Foundation of Chemists Award
University of Oregon
1996

Graduated summa cum laude and with Departmental Honors
University of Oregon
1996