People

David Pincus, PhD

David Pincus is an Assistant Professor in the Dept. of Molecular Genetics and Cell Biology. The Pincus lab is located in the Center for Physics of Evolving Systems on the 5th floor of GCIS.



The Pincus Lab studies "System Mechanics of Cellular Stress Responses", defined as quantitative control processes cells employ to sense and respond to environmental and endogenous perturbations.



David is trained in approaches in biochemistry, biophysics, genetics, and molecular, cell, computational, systems and synthetic biology. The lab uses budding yeast and cultured human cells as experimental models, and applies this toolkit to investigate three interconnected areas:



1) Hsf1, chaperones, and proteostasis networks

2) Stress responsive kinase signaling

3) Stress responsive gene expression



The goal of the research program is to understand the establishment and maintenance of cell-level homeostasis.

Whitehead Institute
Cambridge, MA

2018

UCSF
San Francisco, CA
PhD - Biochemistry
2012

UC Berkeley
Berkeley, CA
BA - Molecular & Cell Biology
2004

Primordial super-enhancers: heat shock-induced chromatin organization in yeast.
Kainth AS, Chowdhary S, Pincus D, Gross DS. Primordial super-enhancers: heat shock-induced chromatin organization in yeast. Trends Cell Biol. 2021 May 14.
PMID: 34001402

Subcellular localization of the J-protein Sis1 regulates the heat shock response.
Feder ZA, Ali A, Singh A, Krakowiak J, Zheng X, Bindokas VP, Wolfgeher D, Kron SJ, Pincus D. Subcellular localization of the J-protein Sis1 regulates the heat shock response. J Cell Biol. 2021 01 04; 220(1).
PMID: 33326013

Persistent Activation of mRNA Translation by Transient Hsp90 Inhibition.
Tsvetkov P, Eisen TJ, Heinrich SU, Brune Z, Hallacli E, Newby GA, Kayatekin C, Pincus D, Lindquist S. Persistent Activation of mRNA Translation by Transient Hsp90 Inhibition. Cell Rep. 2020 Sep 08; 32(10):108149.
PMID: 32905780

Persistent Activation of mRNA Translation by Transient Hsp90 Inhibition.
Tsvetkov P, Eisen TJ, Heinrich SU, Brune Z, Hallacli E, Newby GA, Kayatekin C, Pincus D, Lindquist S. Persistent Activation of mRNA Translation by Transient Hsp90 Inhibition. Cell Rep. 2020 08 11; 32(6):108001.
PMID: 32783929

Multi-kinase control of environmental stress responsive transcription.
Mace K, Krakowiak J, El-Samad H, Pincus D. Multi-kinase control of environmental stress responsive transcription. PLoS One. 2020; 15(3):e0230246.
PMID: 32160258

Regulation of Hsf1 and the Heat Shock Response.
Pincus D. Regulation of Hsf1 and the Heat Shock Response. Adv Exp Med Biol. 2020; 1243:41-50.
PMID: 32297210

Strategies for Engineering and Rewiring Kinase Regulation.
McCormick JW, Pincus D, Resnekov O, Reynolds KA. Strategies for Engineering and Rewiring Kinase Regulation. Trends Biochem Sci. 2020 03; 45(3):259-271.
PMID: 31866305

m6A modification of a 3' UTR site reduces RME1 mRNA levels to promote meiosis.
Bushkin GG, Pincus D, Morgan JT, Richardson K, Lewis C, Chan SH, Bartel DP, Fink GR. m6A modification of a 3' UTR site reduces RME1 mRNA levels to promote meiosis. Nat Commun. 2019 07 30; 10(1):3414.
PMID: 31363087

Proteotoxicity from aberrant ribosome biogenesis compromises cell fitness.
Tye BW, Commins N, Ryazanova LV, Wühr M, Springer M, Pincus D, Churchman LS. Proteotoxicity from aberrant ribosome biogenesis compromises cell fitness. Elife. 2019 03 07; 8.
PMID: 30843788

Heat Shock Factor 1 Drives Intergenic Association of Its Target Gene Loci upon Heat Shock.
Chowdhary S, Kainth AS, Pincus D, Gross DS. Heat Shock Factor 1 Drives Intergenic Association of Its Target Gene Loci upon Heat Shock. Cell Rep. 2019 01 02; 26(1):18-28.e5.
PMID: 30605674

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Stewart Trust Cancer Fellowship
Alexander and Margaret Stewart Trust
2013 - 2015

Early Independence Award (DP5)
NIH Office of the Director
2013 - 2018

Graduate Research Felloship
NSF
2007 - 2010